Analysis Of The Potential Of Natural Sterol Compounds From Tin Ficus carica Leaves as Anti Hypercholesterolemia with In Silico Tests

Abstract

This study aims to determine the potential of natural sterol compounds from Tin Ficus carica< em> leaves as anticholesterolemic using in silico test This study employed an in silico approach using molecular docking methods to evaluate the potential of sterol compounds as anti hypercholesterolemic agents in comparison to other references compounds Data processing and interpretation were conducted using molecular databases for both ligands and target proteins The analysis utilized several computational tools including PyRx 0 8 PyMOL LigPlus and Discovery Studio 2016 Client The compounds used in this research were sterol fluvastatin and simvastatin as control with HMG CoA reductase as the target protein The results showed that the highest binding affinity value was fluvastatin which is 8 3 kcal mol Sterol compounds are compounds with lower binding affinity which is 7 7 kcal mol From the visualization results it is known that the binding distance between sterol compounds with target proteins HMG CoA reductase is between 2 69 to 5 49 and the binding distance between simvastatin compounds with target proteins HMG CoA reductase is between 2 76 to 5 36 and the binding distance between fluvastatin compounds with target proteins HMG CoA reductase is between 1 95 to 5 26 Armstrong While based on the comparison of the binding side of strerol simvastatin and fluvastatin it is known that the three compounds have the same site because they bind to the same amino acid residues namely ARG B: 515 TYR A: 533 TYR B: 533 TYR B: 517 PRO A: 511 The results of this research indicate that the natural sterol compounds found in Tin Ficus carica< em> leaves have potential as anti hypercholesterolemic agents based on reverse docking analysis These sterol compounds bind to the same active site as the control drugs simvastatin and fluvastatin and exhibit comparable binding affinity values< p>