Indonesian Biodiversity Journal

GROWTH ACTIVITY TEST OF WHITE RAT HAIR COMBINATION OF CELERY (Apium Graveolens) LEAF EXTRACT AND GREEN GEDI (Abelmoschus Manihot) LEAF

2025-01-21T09:06:58+00:00

Celery and Green Gedi leaves have been long considered traditional topical supplements to support hair growth. They contain flavanoids that are efficacious to induce keratinocyte proliferation. However, the research about whether the combination of celery and green gedi leaves can still provide an effect is limited. This current study aims to determine the effect of the combination of ethanol extracts of celery leaves and green gedi leaves on white rat hair growth. This is laboratory experimental research to compare the hair growth in three different groups with normal and positive control. Animals were randomly grouped (n=3 for each group) and then given a combination of leaf extracts and carbopol with a concentration of 17.5%:10%; 27.5%:15%;37.5%:20%. A normal control group was left untreated, while another group was treated with 2% minoxidil as positive control. The results of measuring fur weight on day 21 showed that the treatment group given the combination of extracts did affect the growth of rat hair. Statistical tests using one-way ANOVA showed a p-value of <0.001 which means there is a significant difference in the average hair weight of rats treated. LSD post hoc test showed that treatment with a 17.5%:10% concentration ratio was significantly different from the other treatments. It can be concluded that the combination of celery leaf extract and gedi leaf positively affects rat hair growth.

Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus

2024-12-06T08:34:33+00:00

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.

IDENTIFICATION OF MACROALGAE IN THE COASTAL AREA OF BUDO TOURISM VILLAGE, WORI, NORTH MINAHASA

2024-12-30T10:39:30+00:00

Algae are lower plants that do not have differentiated structures such as roots, stems, and leaves; although they may appear to have differences, they are actually just thallus forms. Macroalgae have macroscopic body shape and size consisting of fronds without distinct roots, stems, and leaves. Macroalgal diversity is not only reflected in their ecological and economic significance but also in their high species richness and wide geographical distribution within aquatic ecosystems. However, research on the species diversity of macroalgae in Budo Tourism Village Beach, North Minahasa remains limited. This study aims to identify the macroalgal species in the Budo Tourism Village, Wori, North Minahasa area. The research employed the line transect and quadrat-based sampling method, which was implemented at two distinct station locations: Station I (rocky reef substrate) and Station II (sandy-muddy substrate). Macroalgae identification was facilitated by utilizing the comprehensive identification textbooks. The research reveals the presence of four macroalgal species, comprising three brown algae (Phaeophyceae), namely Padina boergesenii, Sargassum llinearifolium, Turbinaria ornata and one red algae (Rhodophyceae), Gracilaria corticata. Sargassum linearifolium, Padina boergesenii, and Gracilaria corticata was found in rocky reef substrate. Turbinaria ornate and Padina boergesenii was found in sandy-muddy substrate.

Analysis of Weed Vegetation on Paddy Rice (Oryza sativa) Plant in Koya Village, South Tondano District

2024-12-06T08:20:18+00:00

Paddy Rice (Oryza sativa) is the main food commodity in Indonesia, because most of the Indonesian population's staple food is Paddy Rice. Demand for Paddy Rice continues to increase from time to time along with population growth. The aim of this research is to determine the types and control of weeds in lowland Paddy Rice plants (Oryza sativa). This research is a qualitative descriptive study using a survey method directly observing weeds in the Paddy Rice planting area in Koya village, South Tondano subdistrict. The results of research on the density, dominance, and frequency of weed types in lowland Paddy Rice plants in Koya Village, South Tondano District show that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, namely Panicum repens, Fimbristylis litotoralis, Cyperus rotundus, and Pistia stratiotes. P.repens is a grass weed, F.litoralis and C.rotundus are a weed, and P.stratiotes is a broadleaf weed. The type that has the highest relative density (KR) is P. repens, namely 3.33%, followed by F. litoratlis, namely 26.67%, and the lowest is C. rotundus and P. stratiotes, namely 20.0%. Relative Dominance Value The highest (DR) was found in the weed type P. stratiotes, namely 51.56%, then followed by C. rotundus, namely 19.27%, F. litoralis, namely 15.10%, and the lowest, P. repens, namely 14.06%. The highest relative frequency (FR) was in P. repens and C. rotundus, namely 33.33%, followed by F. litoralis, namely 22.22% and the lowest was P. stratiotes, namely 11.11%. Based on the research results, it can be concluded that there are 4 types of weeds in lowland Paddy Rice plants in Koya Village, South Tondano District with the highest weed dominance index value, namely P. stratiotes, namely 27.55787; Weed control can be done chemically, biologically, mechanically and physically.