Isolation and Identification of Lactic Acid Bacteria from Red Dragon Fruit Hylocereus polyrhicus as Exopolysaccharide Producers
DOI:
https://doi.org/10.53682/ibj.v5i1.7730Keywords:
Lactic Acid Bacteria, Hylocereus polyrhiruz, ExopolysaccharidesAbstract
Cherry tomatoes are a type of Red Dragon Fruit that has various benefits including lowering cholesterol levels preventing colon cancer and strengthening the working power of muscles Isolation of LAB isolated from Dragon Fruit as a production material for EPS This study aims to isolate and identify LAB as a producer of EPS from red dragon fruit Hylocereus polyrhiruz< em> which can produce exopolysaccharides This research uses a descriptive research method Data from experimental research in the laboratory obtained 10 LAB isolates namely isolates NG1 NG2 NG3 NG4 NG5 NG6 NG7 NG8 NG9 and NG10 Based on the identification results isolate NG1 has similarities with the genus Lactococcus spherical gram positive nonmotile non spore While isolates NG2 NG3 NG4 NG5 NG6 NG7 NG8 NG9 and NG10 have similarities with the genus Lactobacillus rod form nonmotile gram positive Based on the morphological characteristics which are gram positive catalase negative and non spore The ten isolates of lactic acid bacteria are capable of producing EPS these are the results of EPS acquisition namely: NG1 152 1 mg L NG2 127 9 mg L NG3 134 6 mg L NG4 130 9 mg L NG5 137 mg L NG6 139 2 mg L NG7 204 9 mg L NG8 156 2 mg L NG9 136 4 mg L and NG10 157 3 mg L The highest amount of EPS was isolated NG7 at 204 9 mg L Meanwhile the lowest EPS was isolated NG4 at 130 9 mg L < p>
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